Produce

Shake Flask

Produce small samples at shake flask scale

Shake flask experiments are the ideal next step after microscale screening studies, enabling confirmation of initial expression results under conditions that more closely resemble production processes. They provide a cost-effective and flexible platform for optimizing protein production while generating material for downstream analyses.

At the shake flask scale, recombinant protein production can be evaluated across different host strains, expression conditions, media formulations, and induction strategies. This stage allows researchers to verify protein yield, solubility, and quality before committing resources to bioreactor cultivation. Our shake flask protein production service provides flexible cultivation volumes ranging from 250 mL to 2 L flasks (working volumes 50-400 mL) and utilizes optimized methanol-free, methanol-induced, and hybrid expression protocols to efficiently generate recombinant protein samples for characterization, analysis, and further development.

  • Production of small recombinant protein samples for research and development.
  • Preparation of frozen or lyophilized protein-containing samples for storage and transport.
  • Evaluation of protein expression levels and yield.
  • Assessment of protein quality and integrity by SDS-PAGE.
  • Analysis of protein functionality through activity and functional assays.
  • Evaluation of protein solubility and purification feasibility (on request).
  • Investigation of protein stability during handling and storage (on request).

Services

Evaluate
Feasibility & Analytics Optimize
Strain & Process development Produce
On-demand protein