is a Biotech company founded in 2020 by Claudia Rinnofner. We focus on the production of protein-based (biological) molecules with unique physical and biochemical properties. We are located in Graz at the Zentrum für Wissens- und Technologietransfer in der Medizin (ZWT), Neue Stiftingtalstraße 2 | 8010 Graz, Austria.
Claudia Rinnofner, DI Dr. earned her PhD at Graz University of Technology (TUG) in the research group of Prof. Anton Glieder at the Institute of Molecular Biotechnology. Her doctoral thesis was devoted to the expression of recombinant proteins in Pichia pastoris employing synthetic promoters. Following her PhD she worked at ACIB GmbH (Austrian Centre of Industrial Biotechnology) in an industrial multi-firm project. Claudia Rinnofner has more than 15 years experience as a scientist, working with Pichia pastoris and strain engineering including the recombinant expression of various heterologous proteins.
Alexander Gutmann, DI Dr. has 14 years of experience in biotechnology research and development. He worked for five years in the CDMO industry at Lonza and Arxada. Alexander managed development and optimization of fermentation and downstream processes and organized scale-up to piloting and commercial manufacturing. He has also a strong academic track record in biocatalysis and related fields. He co-authored more than 20 peer reviewed publications..
Alexandra Deibuk, M.Sc. has a bachelor’s degree in Molecular Biology and a master’s degree in Biochemistry and Molecular Biomedicine. She did her masters thesis at Graz University of Technology in collaboration with myBIOS. She is currently working as Lab-Team Leader at myBIOS.
Sandra Flecker, M.Sc. studied Molecular Biology, Biochemistry and Molecular Biomedicine at University of Graz. She joined myBIOS in 2021. Prior to it she was working in a diagnostics company. She is an experienced scientist, specialized in the field of Pichia pastoris.
Lukas Schönleitner pursued Molecular Biology during his undergraduate studies. He is currently in the final stages of completing his master’s thesis in Biotechnology while also holding a part-time position at myBIOS.
Felber, M., Pichler, H., Ruth, C., 2014. Strains and molecular tools for recombinant protein production in Pichia pastoris. Methods Mol. Biol. 1152, 87–111. https://doi.org/10.1007/978-1-4939-0563-8_5
Fürst, M., Kerschbaumer, B., Rinnofner, C., Migglautsch, A., Winkler, M., Fraaije, M., 2019. Exploring the Biocatalytic Potential of a Self-Sufficient Cytochrome P450 from <i>Thermothelomyces thermophila</i> Adv. Synth. Catal. https://doi.org/10.1002/adsc.201900076
Hartner, F.S., Ruth, C., Langenegger, D., Johnson, S.N., Hyka, P., Lin-Cereghino, G.P., Lin-Cereghino, J., Kovar, K., Cregg, J.M., Glieder, A., 2008. Promoter library designed for fine-tuned gene expression in Pichia pastoris. Nucleic Acids Res. 36, e76. https://doi.org/10.1093/nar/gkn369
Hashem, C., Stolterfoht, H., Rinnofner, C., Steinberger, S., Winkler, M., Pichler, H., 2020. Secretion of Pseudomonas aeruginosa Lipoxygenase by Pichia pastoris upon Glycerol Feed. Biotechnol. J. 15, 2000089. https://doi.org/10.1002/biot.202000089
Mellitzer, A., Ruth, C., Gustafsson, C., Welch, M., Birner-Grünberger, R., Weis, R., Purkarthofer, T., Glieder, A., 2014. Synergistic modular promoter and gene optimization to push cellulase secretion by Pichia pastoris beyond existing benchmarks. J. Biotechnol. 191. https://doi.org/10.1016/j.jbiotec.2014.08.035
Näätsaari, L., Mistlberger, B., Ruth, C., Hajek, T., Hartner, F.S., Glieder, A., 2012. Deletion of the pichia pastoris ku70 homologue facilitates platform strain generation for gene expression and synthetic biology. PLoS One 7. https://doi.org/10.1371/journal.pone.0039720
Rinnofner, C., Kerschbaumer, B., Weber, H., Glieder, A., Winkler, M., 2019. Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst. Biocatal. Agric. Biotechnol. 17, 525–528. https://doi.org/10.1016/J.BCAB.2018.12.022
Ruth, C., Buchetics, M., Vidimce, V., Kotz, D., Naschberger, S., Mattanovich, D., Pichler, H., Gasser, B., 2014. Pichia pastoris Aft1 – a novel transcription factor, enhancing recombinant protein secretion. Microb. Cell Fact. 13, 120. https://doi.org/10.1186/s12934-014-0120-5
Ruth, C., Glieder, A., 2010. Perspectives on synthetic promoters for biocatalysis and biotransformation. ChemBioChem 11. https://doi.org/10.1002/cbic.200900761
Ruth, C., Zuellig, T., Mellitzer, A., Weis, R., Looser, V., Kovar, K., Glieder, A., 2010. Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris. Syst. Synth. Biol. 4. https://doi.org/10.1007/s11693-010-9057-0
Stolterfoht, H., Rinnofner, C., Winkler, M., Pichler, H., 2019. Recombinant Lipoxygenases and Hydroperoxide Lyases for the Synthesis of Green Leaf Volatiles. J. Agric. Food Chem. https://doi.org/10.1021/acs.jafc.9b02690