Technology

General Toolbox

Accelerate your protein production

To maximize productivity, the expression strategy must be tailored to the specific requirements of each target protein. Over the years, we have developed a comprehensive Pichia pastoris expression toolbox featuring methanol-free production technologies, encompassing host strains, expression plasmids, promoters, secretion signals, selection markers, chaperones, co-expression systems, and advanced genome engineering tools. Continuously expanded and refined, the toolbox forms the foundation for efficient feasibility studies, strain engineering, and bioprocess development at myBIOS. It can also be licensed to customers seeking to establish or enhance protein production capabilities within their own R&D and manufacturing facilities, enabling the rapid implementation of proven expression solutions across a wide range of applications.

Access the myPichia toolbox

The myPichia platform provides a centralized overview of our complete P. pastoris toolbox and methanol-free expression platform, enabling researchers to rapidly identify the optimal components for recombinant protein production. Users can explore:

  • Host strains (CBS2612 and CBS7435 derivatives)
  • Engineered chassis (Auxotrophic, Protease deficient, ER-up, UPR-up, Cytosol-up)
  • Methanol-free expression systems (Novel processes and Enhanced Methanol-Free strains)
  • Expression plasmids (next generation pPpT4)
  • Promoters and terminators (PDUO1 and classic P. pastoris promoters)
  • Selection markers (antibiotic and auxotrophic)
  • Marker recycling strategies (FLP)
  • Secretion signals (MF-α and more)

Register today to explore the complete myPichia strain and plasmid collection and identify the optimal expression system for your next protein production project → myPichia platform

Designed for Industrial Application

Our toolbox has been developed with industrial implementation in mind, supporting stringent quality, safety, and regulatory requirements.Production strains can be generated without antibiotic resistance markers and tailored to meet specific regulatory and manufacturing standards. Whether applied by myBIOS in feasibility studies, strain engineering, and process development projects, or deployed directly by customers within their own R&D and manufacturing facilities, the myPichia Platform provides a robust foundation for efficient, scalable, and commercially relevant protein production. From industrial enzymes and biopharmaceuticals to food proteins and novel biocatalysts, myPichia enables the rapid identification,optimization, and implementation of expression solutions tailored to the unique requirements of each product and process.

 

 

Technology

Pichia
pastoris Methanol-free
platform General
Toolbox