Optimize

Strain development

We design, engineer, and optimize a Pichia production strain tailored to your specific protein or process requirements. 

A strain development project is typically structured into three work packages (WPs). WP 1 focuses on applying the myBIOS Toolbox, including various strains and regulatory elements, to evaluate expression performance and establish the basis for methanol-free production. During this phase, several expression strategies are tested in parallel and an initial set of clones is screened to identify promising candidates. WP 2 aims to further increase product titres through targeted optimization approaches such as chaperone co-expression (single and/or combinatorial strategies) and signal sequence engineering. WP 3 implements the best engineering strategy in a comprehensive high-throughput screening with the goal of generating a robust and stable production strain. A multicopy strain development approach can also be included upon request. To support reliable scale-up and enable early identification of potential process bottlenecks, the best-performing strains from each work package are evaluated in lab-scale bioreactor cultivations.

Work packages can be tailored individually according to project needs. Additional services, including expression strain modification and characterization as well as protein and enzyme characterization, are available upon request. Explore our range of strains and expression technologies, and combine the tools that best match your production objectives → myPichia Toolbox.

  • Concept design

Our experts evaluate your target sequence(s), review literature, and consider customer-specific and regulatory requirements.

  • Standard and complex constructs

Optimize expression using proven and advanced tools, including codon optimization, promoter and terminator selection, signal sequence screening, helper-factor co-expression, and multicopy integration.

  • Platform strains

Compare performance across different wild type strain backgrounds, auxotrophic strains, protease-deficient strains, chaperone-enhanced chassis, and engineered methanol-free (EMF) strains.

  • Screening and bioreactor cultivation
Identify superior producers through high-throughput screening and evaluate selected strains in lab-scale bioreactors. This enables titre optimization, early identification of scale-up bottlenecks, and provision of samples for in-house characterization.

Services

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Strain & Process development Produce
On-demand protein